Mouse Anti-His-Tag-UNLB, Monoclonal

Catalog Number: SBA-4603-01M
Article Name: Mouse Anti-His-Tag-UNLB, Monoclonal
Biozol Catalog Number: SBA-4603-01M
Supplier Catalog Number: 4603-01M
Alternative Catalog Number: SBA-4603-01M
Manufacturer: SouthernBiotech
Host: Mouse
Category: Antikörper
Application: ELISA, ICC, IP, WB
Conjugation: Unconjugated
Purified Anti-His-Tag for use in ELISA, western blotting, immunoprecipitation, and immunocytochemistry assays.
Clonality: Monoclonal
Clone Designation: [SB194b]
Isotype: IgG1
Form: Purified
Antibody Type: Secondary Antibody
6X His-tag was immunoprecipitated from CHO-K1 cell lysates transfected with GFP - 6X His-tag with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01M). Lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05), and visualized using chemiluminescent detection.
TEV protease / maltose binding protein (MBP) fusion protein with an internal 6X His-tag was resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01M) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05) secondary antibody and chemiluminescent detection.
CHO-K1 cell lysates transfected with GFP - 6X His-tag were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01M) followed by Goat Anti-Mouse IgG(H+L), Human ads-HRP (SB Cat. No. 1031-05) secondary antibody and chemiluminescent detection.