LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX)

Article Name:	LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX)
Biozol Catalog Number:	SBI-LV340A-1
Supplier Catalog Number:	LV340A-1
Alternative Catalog Number:	SBI-LV340A-1
Manufacturer:	System Biosciences
Category:	Molekularbiologie

LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it & TransDux MAX) 10 preps

Overview LentiSuite Basic Kit (includes pPACKH1, PureFection, PEG-it, and TransDux MAX)) 10 preps

Simplify large scale lentivirus production with the LentiSuite Basic Kit

Get the reagents you need for large scale lentivirus production efficiently bundled into a single kit with the LentiSuite Basic Kit. This kit is ideal for fast-moving labs that don’t need quantitative titer information, and supports generation of high-titer lentivirus particles for any application where stable gene delivery and expression is desired. Compatible with all of SBI’s lentivectors and constructs, as well as all other third-generation lentivectors, the LentiSuite Basic Kit includes optimized reagents to support large-scale virus production—you provide the HEK293T/TN/FT producer cells and lentivector (or purchase them separately) and this kit provides enough critical reagents to support 10 virus preps from 10 cm plates, or 5 virus preps from 15 cm plates:

pPACK Packaging System (100 µL)
PureFection Transfection Reagent (120 µL)
PEG-it Concentration Reagent (20 mL)
TransDux™ MAX Transduction Reagent (10 reactions*)

With the LentiSuite Basic Kit, you get more than the kit—you get confidence backed by:

SBI’s well-proven, widely-published lentivirus technology
Optimized reagents that we keep on improving
Deep technical expertise from the scientists that develop our lentivirus production products and perform our lentivirus production services

The Kit includes enough reagents for generating lentivirus from either two 15 cm plates or five 10 cm plates.

*One transduction reaction is defined as 500 µL of total volume in a single well of a 24-well plate

How it works

Kit Contents

pPACK Packaging System

Reliable—generate high titers of transduction-ready pseudoviral particles
Powerful—efficiently deliver and stably express your lentivector constructs in a wide range of mammalian cells
Well-designed—quantify pseudoviral titers and transduction efficiency using a convenient copGFP control
Flexible—choose from FIV and HIV-based lentivector packaging kits

PureFection Transfection Reagent

Flexible—highly effective transfection technology supports a range of applications
Compatible with most cell types
Works with both plasmid DNA and siRNAs
Cost-effective—excellent alternative to expensive, lipid-based products
Safe—nano-based gene delivery with low toxicity
Fast—quick fifteen-minute protocol makes PureFection ideal for high-throughput transfections

PEG-it Concentration Reagent

Easy-to-use—simplifies lentiviral production by removing the need for ultracentrifugation
Cost-effective—contributes to more efficient transductions
Powerful—concentrate lentiviral particles 10- to 100-fold
Scalable—enables isolation of virus from large volumes of media
Safe—supports a range of applications
Found to be non-toxic to all target cell lines tested, including ES cells
Prevent toxic and immunogenic reactions by avoiding accumulation of cellular debris

TransDux™ MAX Transduction Reagent

Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
Non-toxic—no need to change out media after infection
Versatile—works with all types of packaged lentivirus
Flexible—enhances transduction efficiency in a wide variety of cell lines
Broadly compatible—does not interfere with downstream gene expression or functional assays

*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s UltraRapid Titering Kit (Cat #LV961A-1).

Storage:

See Manual