Frozen stock solution of buffer containing: 250mM HEPES, pH7.5, 250mM NaCl, 10mM MgCl2, 2.5mM EGTA, 125µg/mL BSA and 0.2% Triton X-100. Add to final concentration of 0.25mM DTT (Catalog D86-09) to the aliquot prior to use. Supplied in separate vial.
Source:
The 5x stock solution of buffer used for assaying lipid kinase proteins.
Form:
Frozen stock solution of buffer containing: 250mM HEPES, pH7.5, 250mM NaCl, 10mM MgCl2, 2.5mM EGTA, 125μg/mL BSA and 0.2% Triton X-100. Add to final concentration of 0.25mM DTT (Catalog# D86-09) to the aliquot prior to use. Supplied in separate vial.
Formula:
Frozen stock solution of buffer containing: 250mM HEPES, pH7.5, 250mM NaCl, 10mM MgCl2, 2.5mM EGTA, 125g/mL BSA and 0.2% Triton X-100. Add to final concentration of 0.25mM DTT (Catalog D86-09) to the aliquot prior to use. Supplied in separate vial.
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