Non-cleavable K63 polyUb chains are made of the Ub(L73P) mutant, this substitution makes the formed polyubiquitin chains to be resistant to the majority of deubiquitinating enzymes.
Buffer:
20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Source:
E. coli recombinant
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