Non-cleavable linear Ub6 was a derivative of 6xHis-non-cleavable linear Ub6 by removal of the N-terminal 6xHis and 3xHA tags using TEV digestion. The G76V substitution was introduced in the first five Ub moieties and the last Ub has G76.
Molecular Weight:
51 kDa
Buffer:
20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Source:
E. coli recombinant
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