PSG-1, BioAssay(TM) ELISA Kit (Human) (B1G1, CD66f, DHFRP2, PBG1, PSbG1, PSG95, PSGGA)

Product Overview

• Article Name: PSG-1, BioAssay(TM) ELISA Kit (Human) (B1G1, CD66f, DHFRP2, PBG1, PSbG1, PSG95, PSGGA)
• Biozol Catalog Number: USB-145764
• Supplier Catalog Number: 145764
• Alternative Catalog Number: USB-145764-1
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

The PSG-1, Human BioAssay(TM) ELISA Kit Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human PSG-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PSG-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PSG-1 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human PSG-1. Intended Use: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for PSG-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSG-1 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked monoclonal antibody specific for PSG-1 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSG-1 bound in the initial step. The color development is stopped and the intensity of the color is measured. Specificity: Recognizes natural and recombinant human PSG-1. Sensitivity: ~0.48ng/ml Range: ~3.13-200ng/ml (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma) Kit Components: PSG-1 Microplate, 1x96wells PSG-1 Conjugate, 1x21ml PSG-1 Standard, 1x1000ng Assay Diluent, 1x11ml Calibrator Diluent, 1x21ml Wash Buffer Concentrate, 1x21ml Color Reagent A, 1x12ml Color Reagent B, 1x12ml Stop Solution, 1x6ml Plate Sealers, 1x4 adhesive strips Storage and Stability: Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.