RecQ, Recombinant, E. coli, aa2-606, His-Tag (ATP-dependent DNA Helicase RecQ)
Biozol Catalog Number:
USB-375020
Supplier Catalog Number:
375020
Alternative Catalog Number:
USB-375020-20,USB-375020-100,USB-375020-1
Manufacturer:
US Biological
Category:
Molekularbiologie
Involved in the RecF recombination pathway, its gene expression is under the regulation of the SOS system. It is a DNA helicase. Recombinant protein corresponding to aa2-606 from E. coli ATP-dependent DNA Helicase RecQ, fused to 6X His-Tag at N-terminal, expressed in E. coli Swiss/UniProt Accession: P15043 Molecular Weight: ~71.9kD Amino Acid Sequence: AQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRPEYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
