Avidin D, Fluorescein labeled, is made from our highly purified Avidin D by a special labeling technique. This product has an optimal fluorochrome to protein ratio and is the reagent we recommend for routine immunofluorescence applications. Compared to conventional primary and secondary fluorescent labeling techniques, Fluorescein Avidin D can provide greater sensitivity and lower background staining.
Excitation maximum: 495 nm
Emission maximum: 515 nm
Color: Green
Specifications
Unit Size
5 mg
Applications
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage
For diluting this product, we recommend a HEPES- or bicarbonate-buffered saline solution, approximately pH 8.2. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution
10mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Excitation
495-500 nm
Maximum Emission
514-521 nm
Recommended Storage
2-8 °C
Concentration
5.0 mg active conjugate/ml
Conjugate
Fluorescein
Color of Fluorescence
Green
Format
Concentrate
* VAT and and shipping costs not included. Errors and price changes excepted
