Alkaline Phosphatase Avidin D is produced with the highest specific activity enzymes in optimal ratios. It is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.
Features:
Specific covalent linkages are chosen to provide stable, highly active conjugates
One unit is 1 µmole p-nitrophenylphosphate hydrolyzed per min. at 37 °C, pH 9.8
Specifications
Unit Size
100 U
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage
Reconstitute by adding 1 ml water. The resulting solution will have the following composition: 10 mM HEPES, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 0.1 mM zinc acetate, pH 7.5, 50 mg/ml Ficoll, and 2 mg/ml bovine serum albumin. Recommended concentration for use is 0.2 units/ml.
Recommended Storage
2-8 °C
Detection Enzyme(s)
Alkaline Phosphatase
Conjugate
Alkaline Phosphatase
Format
Concentrate
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