Concanavalin A (Con A), Agarose bound, 100 ml

Product Overview

• Article Name: Concanavalin A (Con A), Agarose bound, 100 ml
• Biozol Catalog Number: VEC-AL-1003-100
• Supplier Catalog Number: AL-1003-100
• Alternative Catalog Number: VEC-AL-1003-100
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Concanavalin A (Con A), Agarose bound, 100 ml

Additional Information

Description

Agarose bound Con A is prepared using our affinity-purified lectins. Con A recognizes α-linked mannose present as part of a core oligosaccharide in many serum and membrane glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer of four identical subunits; below pH 5.6, Con A dissociates into active dimers of 52 kDa. Acetylation, succinylation, or other derivatizations can also produce stable forms with dimeric structures. (See succinylated Con A). Nicks in the sequence are often present in the purest preparations due to hydrolytic damage within the seeds.

 

Features:

• Bead diameter ranges in size from 45-165 microns
• Matrix is stable in solutions at pH 3-11 as well as many organic solvents
• Immobilized lectins are prepared using affinity purified lectins
• Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
• Hydrophilic spacer arm is inserted between the lectin and the matrix
• Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
• No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
• Product supplied as a 1:1 suspension in buffer

 

Specifications

• Unit Size: 100 ml
• Applications: Glycobiology, Affinity Chromatography
• Recommended Storage: 2-8 °C DO NOT FREEZE
• Solution: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM glucose, 0.08% sodium azide
• Recommended Usage: Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, a 0.2 M α-methyl mannoside/0.2 M α-methyl glucoside mixture can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
• Matrix Conjugate: Lectins
• Sugar Specificity: Mannose, Glucose
• Conjugate: Agarose