Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 2 ml

Product Overview

• Article Name: Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 2 ml
• Biozol Catalog Number: VEC-AL-1023S-2
• Supplier Catalog Number: AL-1023S-2
• Alternative Catalog Number: VEC-AL-1023S-2
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Succinylated Wheat Germ Agglutinin (WGA), Agarose bound, 2 ml

Additional Information

Description

Agarose bound, succinylated WGA is prepared using our affinity-purified lectins. This derivative has been reported to have properties distinct from the native lectin. Evidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Using conjugates of the native lectin and the succinylated form can provide a system to distinguish between sialylated glycoconjugates and those containing only N-acetylglucosamine structures.

 

Features:

• Bead diameter ranges in size from 45-165 microns
• Matrix is stable in solutions at pH 3-11 as well as many organic solvents
• Immobilized lectins are prepared using affinity purified lectins
• Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
• Product supplied as a 1:1 suspension in buffer
• Inhibiting/Eluting Sugar: Chitin Hydrolysate; or 500 mM N-acetylglucosamine with salt and/or acid elution generally required; or Glycoprotein Eluting Solution (ES-5100)

 

Specifications

• Unit Size: 2 ml
• Applications: Glycobiology, Affinity Chromatography
• Recommended Storage: 2-8 °C DO NOT FREEZE
• Solution: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM GlcNAc, 0.08% sodium azide
• Recommended Usage: Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-5100. Alternatively, 0.5 M N-Acetyl-D-Glucosamine (GlcNAc) can be used.For those glycoconjugates having very high affinity for WGA, it may be necessary to lower the pH of the eluting sugar solution to pH 3.0 with acetic acid and increase the concentration of GlcNAc. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
• Matrix Conjugate: Lectins
• Sugar Specificity: N-Acetylglucosamine
• Conjugate: Agarose