Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml
Biozol Catalog Number:
VEC-AL-1063
Supplier Catalog Number:
AL-1063
Alternative Catalog Number:
VEC-AL-1063
Manufacturer:
Vector Laboratories
Category:
Sonstiges
Ulex Europaeus Agglutinin I (UEA I), Agarose bound, 2 ml
Description
Agarose bound Ulex europaeus agglutinin is prepared using our affinity-purified lectins. Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.
Features:
Bead diameter ranges in size from 45-165 microns
Matrix is stable in solutions at pH 3-11 as well as many organic solvents
Immobilized lectins are prepared using affinity purified lectins
Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
Product supplied as a 1:1 suspension in buffer
Inhibiting/Eluting Sugar: 50 mM - 100 mM L-fucose or Glycoprotein Eluting Solution (ES-3100)
Specifications
Unit Size
2 ml
Applications
Glycobiology, Affinity Chromatography
Recommended Storage
2-8 °C DO NOT FREEZE
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.1 mM CaCl2, 0.08% sodium azide
Recommended Usage
Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate
Lectins
Sugar Specificity
Fucose, Arabinose
Conjugate
Agarose
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