Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound, 2 ml
Biozol Catalog Number:
VEC-AL-1143
Supplier Catalog Number:
AL-1143
Alternative Catalog Number:
VEC-AL-1143
Manufacturer:
Vector Laboratories
Category:
Sonstiges
Erythrina Cristagalli Lectin (ECL, ECA), Agarose bound, 2 ml
Description
Agarose bound Erythrina cristagalli lectin is prepared using 4% agarose beads. Erythrina cristagalli lectin consists of two different subunits of approximately 28 kDa and 26 kDa. The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin.
Features:
Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons
Bead diameter ranges in size from 45-165 microns
Matrix is stable in solutions at pH 3-11 as well as many organic solvents
Immobilized lectins are prepared using affinity purified lectins
Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
Hydrophilic spacer arm is inserted between the lectin and the matrix
Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
No residual charges present after conjugation. This minimizes non-specific binding to the matrix
Product supplied as a 1:1 suspension in buffer
Inhibiting/Eluting Sugar: 200 mM lactose or Glycoprotein Eluting Solution (ES-1100)
Specifications
Unit Size
2 ml
Applications
Glycobiology, Affinity Chromatography
Recommended Storage
2-8 °C DO NOT FREEZE
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 20 mM lactose, 0.08% sodium azide
Recommended Usage
Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 0.2 M lactose can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate
Lectins
Sugar Specificity
Galactose, N-Acetylgalactosamine, Lactose
Conjugate
Agarose
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