Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml

Catalog Number: VEC-AL-1233
Article Name: Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml
Biozol Catalog Number: VEC-AL-1233
Supplier Catalog Number: AL-1233
Alternative Catalog Number: VEC-AL-1233
Manufacturer: Vector Laboratories
Category: Sonstiges
Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml

Description

Agarose bound Vicia villosa lectin is prepared using our affinity-purified lectins. This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen). 

Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine or Glycoprotein Eluting Solution (ES-2100)

 

Specifications

 
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-2100. Alternatively, 200 mM N-acetylgalactosamine can be used.Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity N-Acetylgalactosamine
Conjugate Agarose