Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml
Biozol Catalog Number:
VEC-AL-1233
Supplier Catalog Number:
AL-1233
Alternative Catalog Number:
VEC-AL-1233
Manufacturer:
Vector Laboratories
Category:
Sonstiges
Vicia Villosa Lectin (VVL, VVA), Agarose bound, 2 ml
Description
Agarose bound Vicia villosa lectin is prepared using our affinity-purified lectins. This lectin is a family of tetrameric glycoproteins consisting of combinations of A and B subunits similar in structure to PHA and GSL I. The dominant isolectins in our preparations appear to be B subunit-rich. VVL recognizes preferentially α- or β-linked terminal N-acetylgalactosamine, especially a single α-N-acetylgalactosamine residue linked to serine or threonine in a polypeptide (the Tn antigen).
Features:
Bead diameter ranges in size from 45-165 microns
Matrix is stable in solutions at pH 3-11 as well as many organic solvents
Immobilized lectins are prepared using affinity purified lectins
Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
No residual charges present after conjugation. This minimizes non-specific binding to the matrix
Product supplied as a 1:1 suspension in buffer
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine or Glycoprotein Eluting Solution (ES-2100)
Specifications
Unit Size
2 ml
Applications
Glycobiology, Affinity Chromatography
Recommended Storage
2-8 °C DO NOT FREEZE
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM galactose, 0.08% sodium azide
Recommended Usage
Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting solution, Cat. No. ES-2100. Alternatively, 200 mM N-acetylgalactosamine can be used.Elution can also be achieved with 100 mM sodium acetate, pH 3.0, 1 M galactose. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate
Lectins
Sugar Specificity
N-Acetylgalactosamine
Conjugate
Agarose
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