Galanthus Nivalis Lectin (GNL), Agarose bound, 5 ml

Product Overview

• Article Name: Galanthus Nivalis Lectin (GNL), Agarose bound, 5 ml
• Biozol Catalog Number: VEC-AL-1243-5
• Supplier Catalog Number: AL-1243-5
• Alternative Catalog Number: VEC-AL-1243-5
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Galanthus Nivalis Lectin (GNL), Agarose bound, 5 ml

Additional Information

• Description

  Agarose bound Galanthus nivalis lectin is prepared using our affinity-purified lectins. Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues.

  In contrast to most mannose-binding lectins, GNL will not bind α-linked glucose. Reports indicate that this lectin binds rat and mouse IgM but not IgG. The only protein from human serum reported to bind to this lectin is α2-macroglobulin. GNL binds to many viral glycoproteins.

  Features:

  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation. This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 3 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: 100 mM - 200 mM α-methylmannoside or Glycoprotein Eluting Solution (ES-1100)

  Specifications

  Unit Size	5 ml
  Applications	Glycobiology, Affinity Chromatography
  Recommended Storage	2-8 °C DO NOT FREEZE
  Solution	10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.01 mM MnCl2, 20 mM mannose, 0.08% sodium azide
  Recommended Usage	Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-1100. Alternatively, 0.1 M α methyl mannoside can be used.For those glycoconjugates having a very high affinity for GNL, it may be necessary to lower the pH of the eluting sugar solution to pH 4.0 with acetic acid and increase the concentration of the α methyl mannoside to 0.5 M. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
  Matrix Conjugate	Lectins
  Sugar Specificity	Mannose
  Conjugate	Agarose