Sambucus Nigra Lectin (SNA, EBL), Agarose bound, 2 ml

Product Overview

• Article Name: Sambucus Nigra Lectin (SNA, EBL), Agarose bound, 2 ml
• Biozol Catalog Number: VEC-AL-1303
• Supplier Catalog Number: AL-1303
• Alternative Catalog Number: VEC-AL-1303
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Sambucus Nigra Lectin (SNA, EBL), Agarose bound, 2 ml

Additional Information

Description

Agarose bound Sambucus nigra lectin is prepared using our affinity-purified lectins. Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. 

Features:

• Bead diameter ranges in size from 45-165 microns
• Matrix is stable in solutions at pH 3-11 as well as many organic solvents
• Immobilized lectins are prepared using affinity purified lectins
• Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
• Hydrophilic spacer arm is inserted between the lectin and the matrix
• Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
• No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
• Product supplied as a 1:1 suspension in buffer
• 3 mg lectin/ml gel
• 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid or Glycoprotein Eluting Solution (ES-7100)

 

Specifications

• Unit Size: 2 ml
• Applications: Glycobiology, Affinity Chromatography
• Recommended Storage: 2-8 °C DO NOT FREEZE
• Solution: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.1 mM CaCl2, 0.08% sodium azide, 20 mM lactose
• Recommended Usage: Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-7100. Alternatively, 0.5 M lactose in buffered saline followed by 0.5 M lactose in 0.2 M acetic acid can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline, containing 0.08% sodium azide for storage.
• Matrix Conjugate: Lectins
• Sugar Specificity: Sialic Acid
• Conjugate: Agarose

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