Aleuria Aurantia Lectin (AAL), Agarose bound, 2 ml

Catalog Number: VEC-AL-1393
Article Name: Aleuria Aurantia Lectin (AAL), Agarose bound, 2 ml
Biozol Catalog Number: VEC-AL-1393
Supplier Catalog Number: AL-1393
Alternative Catalog Number: VEC-AL-1393
Manufacturer: Vector Laboratories
Category: Sonstiges
Aleuria Aurantia Lectin (AAL), Agarose bound, 2 ml

Description

Features:

  • Matrix is heat stable, cross-linked 4% agarose beads with a molecular exlusion of about 2x107 daltons
  • Bead diameter ranges in size from 45-165 microns
  • Matrix is stable in solutions at pH 3-11 as well as many organic solvents
  • Immobilized lectins are prepared using affinity purified lectins
  • Covalent attachment preserves lectin activity and minimizes conformational changes that might result in nonspecific or hydrophobic interactions
  • Hydrophilic spacer arm is inserted between the lectin and the matrix
  • Conjugated proteins are not leached off the beads by Tris or other routinely used buffers
  • No residual charges present after conjugation.  This minimizes non-specific binding to the matrix
  • Product supplied as a 1:1 suspension in buffer
  • 2 mg lectin/ml gel
  • Inhibiting/Eluting Sugar: 100 mM L-fucose or Glycoprotein Eluting Solution (ES-3100)

 

Specifications

 
Unit Size 2 ml
Applications Glycobiology, Affinity Chromatography
Recommended Storage 2-8 °C DO NOT FREEZE
Solution 10 mM HEPES, pH 7.5, 0.15 M NaCl, 10 mM fucose, 0.08% sodium azide
Recommended Usage Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-3100. Alternatively, 100 mM L-fucose in buffered saline can be used. After use, wash the gel with several column volumes of buffered saline then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Matrix Conjugate Lectins
Sugar Specificity Fucose, Arabinose
Conjugate Agarose