Wheat Germ Agglutinin (WGA), Biotinylated, 5 mg

Product Overview

• Article Name: Wheat Germ Agglutinin (WGA), Biotinylated, 5 mg
• Biozol Catalog Number: VEC-B-1025
• Supplier Catalog Number: B-1025
• Alternative Catalog Number: VEC-B-1025
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Wheat Germ Agglutinin (WGA), Biotinylated, 5 mg

Additional Information

Description

The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. 

Biotinylated WGA has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

 

Specifications

• Unit Size: 5 mg
• Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
• Recommended Usage: For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
• Recommended Storage: 2-8 °C; Store frozen for long term storage
• Solution: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 10 mM N-acetylglucosamine.
• Concentration: 5 mg active conjugate/ml
• Conjugate: Biotinylated
• Sugar Specificity: N-Acetylglucosamine