Lens Culinaris Agglutinin (LCA), Biotinylated, 5 mg

Product Overview

• Article Name: Lens Culinaris Agglutinin (LCA), Biotinylated, 5 mg
• Biozol Catalog Number: VEC-B-1045
• Supplier Catalog Number: B-1045
• Alternative Catalog Number: VEC-B-1045
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Lens Culinaris Agglutinin (LCA), Biotinylated, 5 mg

Additional Information

Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity.  By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Biotinylated Lens culinaris agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.

 

Specifications

• Unit Size: 5 mg
• Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology
• Recommended Usage: For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer.
• Recommended Storage: 2-8 °C; Store frozen for long term storage
• Solution: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM CaCl2, 0.01 mM MnCl2, 0.08% sodium azide, 10 mM methyl-alpha-D-mannopyranoside.
• Concentration: 5 mg active conjugate/ml
• Conjugate: Biotinylated
• Sugar Specificity: Mannose, Glucose