Biotinylated Anti-Streptavidin has been widely used as an amplifying reagent in immunohistochemistry, in situ hybridization, microarray assays, ELISAs, blots, and many other applications. The capability of binding streptavidin via either biotin binding sites or through antigen binding sites, makes this biotinylated antibody unique. Anti-Streptavidin does not bind avidin, and Anti-Avidin does not recognize streptavidin.
Specifications
Unit Size
0.5 mg
Applications
Immunofluorescence, In situ hybridization
Concentration
0.5 mg active conjugate/ml
Recommended Storage
2-8 °C; Store frozen for long term storage
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended Usage
The recommended concentration range for use is 1-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Detection Target
Streptavidin
Conjugate
Biotinylated
Host Species
Goat
Format
Concentrate
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