The biotinylated rabbit anti-goat IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
Recognizes both heavy and light chains (H+L)
Biotinylated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody
Included in the VECTASTAIN® ABC kits
Supplied in solution
Suitable for use with goat, sheep, and bovine IgG primary antibodies
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin.
Recommended Usage
The recommended concentration range for use is 2-10 µg/ml.If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in these applications, blocking with Animal-Free Blocker™ solution (Cat. No. SP-5030) is recommended. Note:This product has the same concentration as that provided in the VECTASTAIN® ABC kits.
Target Species
Goat
Conjugate
Biotinylated
Host Species
Rabbit
Format
Concentrate
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