VectaCell Trolox Antifade Reagent for Live Cell Imaging, 2 ml
Biozol Catalog Number:
VEC-CB-1000
Supplier Catalog Number:
CB-1000
Alternative Catalog Number:
VEC-CB-1000
Manufacturer:
Vector Laboratories
Category:
Sonstiges
VectaCell Trolox Antifade Reagent for Live Cell Imaging, 2 ml
Description
VectaCell Trolox is an antifading additive for live cell imaging. It is a stock solution of the antioxidant Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) in filtered ethanol. Trolox is a water-soluble and cell-permeable analog of vitamin E that efficiently prevents formation of various reactive oxygen species, such as singlet oxygen (1O2), superoxide anion (O2-) or hydrogen peroxide (H2O2).
Specifications
Unit Size
2 ml
Applications
Immunofluorescence, Cellular Imaging
Concentration
100 mM (in ethanol)
Recommended Usage
Instructions for use: Dilute the Trolox Solution into culture media or imaging buffer to a final concentration between 0.1 mM and 1 mM. Notes: The optimal working concentration of Trolox in imaging media will depend on the cell type and their tolerance to hypoxia. For cells that have low tolerance to hypoxia, or can be affected by the addition of ethanol, the appropriate controls should be included in the assay.
Recommended Storage
2-8 °C
Microscopy
Fluorescence
Antifade
Yes
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