DyLight 549 Horse Anti-Mouse IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.
Features:
Recognizes both heavy and light chains (H+L)
Optimally labeled with DyLight™ 549 to provide the brightest label for fluorescence microscopy
Supplied in solution
Excitation: 556 nm
Emission: 571 nm
Color: Orange
Specifications
Unit Size
1.5 mg
Applications
Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration
1.5 mg active conjugate/ml
Recommended Storage
2-8 °C
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission
571 nm
Maximum Excitation
556 nm
Recommended Usage
Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species
Mouse
Conjugate
DyLight 549
Color of Fluorescence
Orange
Host Species
Horse
Format
Concentrate
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