DyLight 488 Horse Anti-Goat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.
Features:
Affinity-purified, ultrapure, high affinity antibody
Suitable for use with goat, sheep, and bovine IgG primary antibodies
Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
Recognizes both heavy and light chains (H+L)
Optimally labeled with DyLight™ 488 to provide the brightest label for fluorescence microscopy
Supplied in solution
Excitation: 493 nm
Emission: 518 nm
Color: Green
Specifications
Unit Size
1.5 mg
Applications
Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration
1.5 mg active conjugate/ml
Recommended Storage
2-8 °C
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission
518 nm
Maximum Excitation
493 nm
Recommended Usage
Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species
Goat
Conjugate
DyLight 488
Color of Fluorescence
Green
Host Species
Horse
Format
Concentrate
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