DyLight 594 Horse Anti-Goat IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.
Features:
Affinity-purified, ultrapure, high affinity antibody
Suitable for use with goat, sheep, and bovine IgG primary antibodies
Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
Recognizes both heavy and light chains (H+L)
Optimally labeled with DyLight™ 594 to provide the brightest label for fluorescence microscopy
Supplied in solution
Excitation: 592 nm
Emission: 617 nm
Color: Red
Specifications
Unit Size
1.5 mg
Applications
Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration
1.5 mg active conjugate/ml
Recommended Storage
2-8 °C
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission
617 nm
Maximum Excitation
592 nm
Recommended Usage
Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue.
Target Species
Goat
Conjugate
DyLight 594
Color of Fluorescence
Red
Host Species
Horse
Format
Concentrate
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