Lens culinaris agglutinin (LCA) recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this increased specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.
DyLight™ 649 labeled LCA has an appropriate number of bound fluorochromes, which provides the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
Excitation maximum: 655 nm
Emission maximum: 670 nm
Color: Far red
Lens Culinaris Agglutinin (LCA), DyLight™ 649 is provided in a 1 mL aliquot at a concentration of 1 mg active conjugate/ml.
Specifications
Unit Size
1 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage
2-8 °C
Maximum Excitation
655 nm
Maximum Emission
670 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2
Concentration
1 mg active conjugate/ml
Conjugate
DyLight 649
Color of Fluorescence
Far Red
Sugar Specificity
Mannose, Glucose
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