Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins, 100 ml
Biozol Catalog Number:
VEC-ES-2100
Supplier Catalog Number:
ES-2100
Alternative Catalog Number:
VEC-ES-2100
Manufacturer:
Vector Laboratories
Category:
Sonstiges
Glycoprotein Eluting Solution for Galactose/GalNAc Binding Lectins, 100 ml
Description
Features:
Convenient, ready-to-use solutions
Superior recovery over standard sugar solutions
Maximize the yield of eluted glycoproteins
Preserve the activity of the agarose-bound lectins for repeated use
Elution in neutral pH range
All components can be subsequently removed by dialysis
Specifications
Unit Size
100 ml
Applications
Glycobiology, Affinity Chromatography
Recommended Usage
1)Wash lectin agarose column to remove unbound proteins with HEPES- or TRIS-Buffered saline (HBS, TBS), pH 7.5, until OD280nm reaches background level. 2)Apply Glycoprotein Eluting Solution and allow to flow through the column by gravity.Note: Do not apply pressure to speed elution because this may reduce the percent recovery of the bound glycoprotein.Complete elution may take up to 10 column volumes.* 3) Following elution, the agarose lectin column is ready for reuse after equilibrating with 2-4 column volumes of HBS or TBS, pH 7.5. *These results may not be typical for other glycoproteins, since oligosaccharide structures of glycoproteins vary significantly.The eluting solution has a high osmolarity.If downstream applications are adversely affected by salt, eluted glycoproteins may require dialysis or gel filtration.
Recommended Storage
Room Temperature
* VAT and and shipping costs not included. Errors and price changes excepted
