Glycoprotein Eluting Solution for Sialic Acid Binding Lectins, 100 ml

Product Overview

• Article Name: Glycoprotein Eluting Solution for Sialic Acid Binding Lectins, 100 ml
• Biozol Catalog Number: VEC-ES-7100
• Supplier Catalog Number: ES-7100
• Alternative Catalog Number: VEC-ES-7100
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Glycoprotein Eluting Solution for Sialic Acid Binding Lectins, 100 ml

Additional Information

Description

Vector Laboratories has developed Glycoprotein Elution Solutions in the neutral pH range that maximize the yield of eluted glycoproteins and preserve the activity of the agarose-bound lectins for repeated use. These Glycoprotein Eluting Solutions offer researchers convenience and superior recovery over standard sugar solutions.

Features:

• All components can be subsequently removed by dialysis

 

Specifications

• Unit Size: 100 ml
• Applications: Glycobiology, Affinity Chromatography
• Recommended Usage: 1)Wash lectin agarose column to remove unbound proteins with HEPES- or TRIS-Buffered saline (HBS, TBS), pH 7.5, until OD280nm reaches background level. 2)Apply Glycoprotein Eluting Solution and allow to flow through the column by gravity.Note: Do not apply pressure to speed elution because this may reduce the percent recovery of the bound glycoprotein.Complete elution may take up to 10 column volumes.* 3) Following elution, the agarose lectin column is ready for reuse after equilibrating with 2-4 column volumes of HBS or TBS, pH 7.5. *These results may not be typical for other glycoproteins, since oligosaccharide structures of glycoproteins vary significantly.The eluting solution has a high osmolarity.If downstream applications are adversely affected by salt, eluted glycoproteins may require dialysis or gel filtration.
• Recommended Storage: Room Temperature

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