Fluorescein Horse Anti-Mouse IgG Antibody can be used for tissue staining or other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.
Features:
Affinity-purified, ultrapure, high affinity antibody
Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
Recognizes both heavy and light chains (H+L)
Optimally labeled with fluorescein to provide the brightest label for fluorescence microscopy
Supplied in solution
Excitation: 495 nm
Emission: 515 nm
Color: Green
Specifications
Unit Size
1.5 mg
Applications
Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation
Concentration
1.5 mg active conjugate/ml
Recommended Storage
2-8 °C
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Emission
510-520 nm
Maximum Excitation
490-500 nm
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2 % normal serum from the same species as the tissue.
Target Species
Mouse
Conjugate
Fluorescein
Color of Fluorescence
Green
Host Species
Horse
Format
Concentrate
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