The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).
Fluorescein labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Specifications
Unit Size
10 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage
2-8 °C
Maximum Excitation
495-500 nm
Maximum Emission
514-521 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration
5 mg active conjugate/ml
Conjugate
Fluorescein
Color of Fluorescence
Green
Sugar Specificity
N-Acetylglucosamine
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