Lens Culinaris Agglutinin (LCA), Fluorescein, 5 mg

Product Overview

• Article Name: Lens Culinaris Agglutinin (LCA), Fluorescein, 5 mg
• Biozol Catalog Number: VEC-FL-1041
• Supplier Catalog Number: FL-1041
• Alternative Catalog Number: VEC-FL-1041
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Lens Culinaris Agglutinin (LCA), Fluorescein, 5 mg

Additional Information

Description

LCA recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this narrower specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

Fluorescein labeled LCA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.

 

Specifications

• Unit Size: 5 mg
• Applications: Immunofluorescence, Glycobiology
• Recommended Usage: The recommended concentration range for use is 5-20 µg/ml.
• Recommended Storage: 2-8 °C
• Maximum Excitation: 495-500 nm
• Maximum Emission: 514-521 nm
• Solution: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2.
• Concentration: 5 mg active conjugate/ml
• Conjugate: Fluorescein
• Color of Fluorescence: Green
• Sugar Specificity: Mannose, Glucose

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