Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Fluorescein, 5 mg
Biozol Catalog Number:
VEC-FL-1101-5
Supplier Catalog Number:
FL-1101-5
Alternative Catalog Number:
VEC-FL-1101-5
Manufacturer:
Vector Laboratories
Category:
Sonstiges
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Fluorescein, 5 mg
Description
GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed A and B, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
Fluorescein labeled GSL I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Specifications
Unit Size
5 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage
2-8 °C
Maximum Excitation
495-500 nm
Inhibiting and/or Eluting Sugar
Mixture of 200 mM galactose (S-9003)/200 mM N-acetylgalactosamine (S-9001).
Maximum Emission
514-521 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration
FL-1101-5 (5 mg active conjugate/ml)
Conjugate
Fluorescein
Color of Fluorescence
Green
Sugar Specificity
Galactose, N-Acetylgalactosamine
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