Erythrina cristagalli lectin (ECL) has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol available upon request or on our website). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose.
Fluorescein labeled Erythrina cristagalli lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
Specifications
Unit Size
5 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage
2-8°C
Maximum Excitation
495-500 nm
Inhibiting and/or Eluting Sugar
200 mM lactose (S-9004)
Maximum Emission
514-521 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration
5 mg active conjugate/ml
Conjugate
Fluorescein
Color of Fluorescence
Green
Sugar Specificity
Galactose, N-Acetylgalactosamine, Lactose
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