SBA preferentially binds to oligosaccharide structures with terminal α- or β-linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B substance.
Although many buffers can be employed for reconstituting and diluting this lectin, we recommend 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Recommended Storage
2-8 °C
Conjugate
Unconjugated
Sugar Specificity
Galactose, N-Acetylgalactosamine
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