The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.
Specifications
Unit Size
5 mg
Applications
Glycobiology, Mitogenic Stimulation
Recommended Usage
Though many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Recommended Storage
2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C
Conjugate
Unconjugated
Sugar Specificity
[GlcNAc]1-3, N-Acetylglucosamine
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