Galanthus nivalis lectin, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (α-1,3) mannose residues.
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. Lectin may be preserved with 0.04% sodium azide.
Recommended Storage
2-8 °C
Conjugate
Unconjugated
Sugar Specificity
Mannose
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