Nucleic acid probes are frequently labeled with digoxigenin (DIG), a steroid found in plants. This is particularly common in double-label in situ hybridization applications with a biotin-labeled probe. DIG-labeled probes can be visualized using our high affinity, purified antibody against digoxigenin/digoxin.
Specifications
Unit Size
1 mg
Applications
In situ hybridization, Blotting Applications
Concentration
1 mg active IgG/ml
Recommended Storage
2-8 °C
Solution
10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose.
Recommended Usage
This product is recommended for use in transfer blot applications (Northern, Southern, Western, dot blots, etc.) and in situ hybridization. The recommended concentration range for use is 0.5-5 µg/ml.This reagent can be detected using a number of anti-goat IgG secondary antibody options.
Detection Target
Digoxigenin/Digoxin (DIG)
Conjugate
Unconjugated
Host Species
Goat
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