Affinity-purified, ultrapure, high affinity antibody
Suitable for use with goat, sheep, and bovine IgG primary antibodies
Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L)
Cross-linked with the highest specific activity horseradish peroxidase
Supplied in solution
Specifications
Unit Size
1 mg
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Concentration
1 mg active conjugate/ml
Recommended Storage
2-8 °C, DO NOT FREEZE
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, preservative
Recommended Usage
Important Note:The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of anti-goat IgG with bovine immunoglobulins that may be present.If this antibody is to be used in thse applications, blocking with Animal-Free blocker™ solution (Cat. No. SP-5030) is recommended. Recommended diluent for microtitration plate assays: 10 mM phosphate, 0.15 M NaCl, pH 7.5, 0.1% Tween 20.Working concentration of HRP-anti goat IgG is 0.5-5.0 µg/ml.
Target Species
Goat
Conjugate
Peroxidase
Host Species
Horse
Format
Concentrate
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