The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).
Rhodamine labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.
Specifications
Unit Size
5 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
For most applications we recommend a freshly prepared working solution of 5-20 µg/mlin the above buffer.
Recommended Storage
2-8 °C
Maximum Excitation
545-555 nm
Inhibiting and/or Eluting Sugar
400 mM N-acetylglucosamine (S-9002)
Maximum Emission
570-580 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 25 mM N-acetylglucosamine
Concentration
5 mg active conjugate/ml
Conjugate
Rhodamine
Sugar Specificity
N-Acetylglucosamine
* VAT and and shipping costs not included. Errors and price changes excepted
