Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Rhodamine, 2 mg
Biozol Catalog Number:
VEC-RL-1102
Supplier Catalog Number:
RL-1102
Alternative Catalog Number:
VEC-RL-1102
Manufacturer:
Vector Laboratories
Category:
Sonstiges
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Rhodamine, 2 mg
Description
GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
Rhodamine labeled GSL I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.
Specifications
Unit Size
2 mg
Applications
Immunofluorescence, Glycobiology
Recommended Usage
The recommended concentration range for use is 5-20 µg/ml.
Recommended Storage
2-8 °C
Maximum Excitation
545-555 nm
Maximum Emission
570-580 nm
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Concentration
2 mg active conjugate/ml
Conjugate
Rhodamine
Color of Fluorescence
Red
Sugar Specificity
Galactose, N-Acetylgalactosamine
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