Protein ̶ Oligonucleotide Conjugation Kit, 1 Kit

Product Overview

• Article Name: Protein ̶ Oligonucleotide Conjugation Kit, 1 Kit
• Biozol Catalog Number: VEC-S-9011-1
• Supplier Catalog Number: S-9011-1
• Alternative Catalog Number: VEC-S-9011-1
• Manufacturer: Vector Laboratories
• Category: Kits/Assays

Product Description

Protein ̶ Oligonucleotide Conjugation Kit, 1 Kit

Additional Information

Description

The Protein-Oligo Conjugation Kit is designed to easily and efficiently prepare two separate protein-oligo conjugates. This kit is flexible so that researchers with little or no conjugation experience can make their own custom protein-oligo conjugates to suit their research needs. It includes all of the necessary components, including S-HyNic and S-4FB, for the rapid and specific crosslinking of any protein with any oligo from 20 to 100 nucleotides in length.

The molecular weight range for proteins to be conjugated with this kit is 25,000 to 900,000 Daltons. Protein amounts from 50 – 650 µg at a concentration of 1 – 5 mg/mL in a volume of 50 – 130 µL are suitable for conjugation.

 

Specifications

• Conjugation Targets: Protein
• Label/Modifier Type: Oligonucleotide
• Reactivity: Amine
• Recommended Storage: 2° - 8°C – Do Not Freeze
• Applications: Antibody Labeling, In Situ Proximity Ligation

 

Kit Components

• S-HyNic (2 X 1 mg)
• S-4FB (2 x 1mg)
• 10X Modification Buffer (1.5 mL)
• 10X Conjugation Buffer (1.5 mL)
• 10X TurboLINK™ Catalyst Buffer (1.5 mL)
• 7kDa MWCO, 0.5 mL Zeba™ Column (4)
• Anhydrous DMF (1.5 mL)
• 2-Hydrazinopyridine (2-HP) (0.5 mL)
• 2-Sulfobenzaldehyde (2-SB) (0.5 mL)
• 7kDa MWCO 2 mL Zeba™ Column (2)
• 10X PBS (1.5 mL)
• 2 mL Collection Tube (12)
• Oligo Resuspension Solution (1.0 mL)