Streptavidin, fluorescein (FITC) can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry. Amplification of fluorescent signals can be easily achieved with our highly purified fluorescein labeled streptavidin.
Features:
Recommended for routine immunofluorescence applications
Extremely high affinity for biotin
Has an optimal fluorochrome to protein ratio
Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining
Excitation maximum at 495 nm
Emission maximum at about 515 nm
Color: Green
Specifications
Unit Size
1 mg
Applications
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage
For diluting this product, we recommend a HEPES-, bicarbonate- or phosphate- buffered saline solution, approximately pH 8.2. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents. The recommended range of concentrations for staining of cells or tissues is 5-30 µg/ml.
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation
495-500 nm
Maximum Emission
514-521 nm
Recommended Storage
2-8 °C
Concentration
1.0 mg active conjugate/ml
Conjugate
Fluorescein
Color of Fluorescence
Green
Format
Concentrate
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