Streptavidin, AMCA, 1 mg

Product Overview

• Article Name: Streptavidin, AMCA, 1 mg
• Biozol Catalog Number: VEC-SA-5008
• Supplier Catalog Number: SA-5008
• Alternative Catalog Number: VEC-SA-5008
• Manufacturer: Vector Laboratories
• Category: Sonstiges

Product Description

Streptavidin, AMCA, 1 mg

Additional Information

Description

AMCA Streptavidin is produced by conjugating streptavidin with a coumarin fluorescent dye, 7-amino-4-methylcoumarin-3-acetic acid. This derivative excites in the ultraviolet (350 nm) and emits in the visible (450 nm) producing an intense blue fluorescence.

Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.

Features:

• Recommended for routine immunofluorescence applications
• Highly purified and possesses very low non-specific binding properties
• Extremely high affinity for biotin
• Has a high fluorochrome to protein ratio
• Compared to conventional primary and secondary fluorescent techniques, can provide greater sensitivity and lower background staining

 

Specifications

• Unit Size: 1 mg
• Applications: Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
• Recommended Usage: For diluting this product, we recommend a HEPES- or biocarbonate-buffered saline solution, approximately pH 8.2.Avoid using RPMI 1640 or other biotin-containing solutions as diluents.Serum also can contain biotin and should not be added to diluents. The recommended concentration range for use is 10-30 µg/ml.
• Solution: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
• Maximum Excitation: 345-355 nm
• Maximum Emission: 448-454 nm
• Recommended Storage: 2-8 °C
• Concentration: 1.0 mg/ml
• Conjugate: AMCA
• Color of Fluorescence: Blue
• Format: Concentrate

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