Alkaline Phosphatase Streptavidin (AP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of alkaline phosphatase. Using biotinylated primary or secondary intermediates and alkaline phosphatase-conjugated streptavidin, it is suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.
Features:
Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios
Specific covalent linkages are chosen to provide stable, highly active conjugates
Specifications
Unit Size
1 ml
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Recommended Usage
The recommended dilution for use is 1:200 to 1:1000.(1-5 µg/ml). Storage of dilute solutions of the enzyme conjugate is not recommended.To avoid adsorption or denaturing of the enzyme conjugate, dilutions may be made in 10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1mg/ml immunohistochemical grade bovine serum albumin (Catalog No. SP-5050).DO NOT USE FRACTION V GRADE BSA.
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 1mM MgCl2, 1% sucrose, 2 mg/ml bovine serum albumin.
Recommended Storage
2-8 °C
Detection Enzyme(s)
Alkaline Phosphatase
Conjugate
Alkaline Phosphatase
Format
Concentrate
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