DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
Features:
Advantages of DyLight™ dyes include greater photostability, pH independence, and brighter fluorescence
Excitation maximum: 493 nm
Emission maximum: 518 nm
Color: Green
Specifications
Unit Size
1 mg
Applications
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage
Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Maximum Excitation
493 nm
Maximum Emission
518 nm
Recommended Storage
2-8 °C
Concentration
1.0 mg/ml active conjugate
Conjugate
DyLight 488
Color of Fluorescence
Green
Format
Concentrate
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