TMB (3,3', 5,5' tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications. Slides stained with TMB can be dehydrated and permanently mounted and the soluble blue product, produced in the absence of the stabilizing solution, is useful for ELISA assays.
The TMB HRP Substrate Kit (SK-4400) contains the reagents needed to produce either a precipitating blue reaction product or a soluble blue reaction product. This kit also contains a stabilizing solution which, when added to the working solution, allows the development of a blue precipitate. Kit materials are pre-packaged in convenient dropper bottles.
Features:
Greater sensitivity than conventional substrates
Stock solutions supplied in convenient dropper bottles promoting ease of handling
No wait times for mixing and dissolving powders or tablets
Sufficient reagents to produce 300 ml of working solution
Specifications
Unit Size
1 kit
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization, Blotting Applications, Elispot, ELISAs
Detection Enzyme(s)
Peroxidase
Substrate Reaction Product
Precipitating Reaction Product
Chromogen Color
Blue
Microscopy
Brightfield, Spectral Imaging
Mounting
Non-Aqueous (Permanent)
Kit Components
What's in the box:
6 ml TMB Reagent 1
6 ml TMB Reagent 2
6 ml TMB Reagent 3
6 ml TMB Reagent 4
* VAT and and shipping costs not included. Errors and price changes excepted
