BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium), 1 Kit

Product Overview

• Article Name: BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium), 1 Kit
• Biozol Catalog Number: VEC-SK-5400
• Supplier Catalog Number: SK-5400
• Alternative Catalog Number: VEC-SK-5400
• Manufacturer: Vector Laboratories
• Category: Kits/Assays

Product Description

BCIP/NBT Substrate Kit, Alkaline Phosphatase (AP), (5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium), 1 Kit

Additional Information

Description

BCIP/NBT AP Substrate Kit forms an indigo, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

Features:

• Greater sensitivity than conventional substrates
• Consistent and reliable
• Ideal for IHC/ICC/ISH and blots
• Fluorescent
• Heat Stable
• Permanent mounting
• No wait times for mixing and dissolving powders or tablets
• Sufficient reagents to produce 200 ml of working solution
• Excitation: 645-685 nm*
• Emission: peaks at 823 and 855 nm*

*Biotechniques (2007) 42:756-759 and BioTechniques (2014) 57:254-256

 

Specifications

• Unit Size: 1 kit
• Applications: Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
• Detection Enzyme(s): Alkaline Phosphatase
• Substrate Reaction Product: Precipitating Reaction Product
• Chromogen Color: Indigo
• Color of Fluorescence: Far Red
• Microscopy: Brightfield, Fluorescence, Spectral Imaging
• Additional Substrate Properties: Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
• Mounting: Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)

 

 

Kit Components

Kit Contents:

• 4 ml BCIP/NBT Reagent 1
• 3 ml BCIP/NBT Reagent 2
• 4 ml BCIP/NBT Reagent 3

Images