The Quick-RNA Viral Kit is a quick viral RNA purification kit for viral RNA from plasma, serum, cell culture media, cellular suspensions, urine, blood, saliva and any other biological samples stored in DNA/RNA Shield™. DNA/RNA Shield™ ensures nucleic acid stability during sample storage/transport at ambient temperatures (4-25°C). The reagent effectively lyses cells and inactivates nucleases and infectious agents (virus). The kit also features a specialized buffer system that facilitates complete viral particle lysis for efficient RNA isolation from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, parvovirus (a ssDNA virus), etc. Viral RNA is bound to the column, washed and eluted. The isolated high-quality viral RNA is ready for all downstream applications such as Next-Gen Sequencing, hybridization-based and RT/PCR detection.
Technical Specifications
| Equipment |
Microcentrifuge |
| Purity |
RNA is ready for Next-Gen sequencing, RT-qPCR, microarray, hybridization, etc. |
| Sample Source |
Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, and swab and fecal samples stored in DNA/RNA Shield |
| Yield |
10 µg RNA (binding capacity), ≥6 µl (elution volume) |
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