Cell Fractionation

Characterizing B cells and Their Antibodies using Single-Cell RNA Sequencing (scRNA-seq)

The study of single cells has provided insights into the composition and function of an increasing number of biological systems across both health and disease. Single-cell RNA sequencing (scRNA-seq) is a particularly powerful approach that can provide the subtype, transcriptional state, and antibody sequence of single B cells.

Monoclonal antibodies derived from these single B cells can then be cloned and characterized, thereby coupling the specificity of each B cell with its transcriptional program.

This webinar covers:

• Current methods of scRNA-seq
• Bioinformatics approaches for identifying antibody sequences within scRNA-seq data
• Single cell monoclonal antibody generation
• Novel insights into food allergy revealed by coupling antibody specificity with transcriptomics