Immunohistochemistry

Tissue Staining with VIP Peroxidase Substrate

Vector® VIP and ImmPACT™ VIP substrates fr4om Vector Laboratories both produce intense, violet reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector VIP and ImmPACT VIP substrates also provide excellent color contrast in pigmented tissues such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector® VIP and ImmPACT™ VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted. The Vector® VIP Substrate kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector® VIP substrate is equivalent to Vector® DAB Substrate Kit (SK-4100).

Learn more about tissue staining with Vector® VIP Peroxidase Substrate in this Vector Laboratories video tutorial.